It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Preferential solvation bromophenol blue in water-alcohol binary mixture. © 1998-2020 Abcam plc. Please store the product under the recommended conditions in the Certificate of Analysis. Migrates at approximately the same rate as 300-500bp DNA… Bromophenol blue, Tracking dye (CAS 115-39-9) (ab146339) | Abcam It is used as a laboratory indicator, changing from yellow below pH 3 to purple at pH 4.6, and as a size marker for monitoring the progress of agarose gel and polyacrylamide gel electrophoresis. Bromophenol is also used as a colour marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. Publishing research using ab146339? Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6[1][2]. Solutions of the dye, therefore, are blue. Room temperature in continental US; may vary elsewhere. Bromphenol Blue - Use and Manufacturing. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. We do not sell to patients. To our knowledge, customised protocols are not required for this product. Submission failed, please try again later. If incorrect, please enter your country/region into the box below, to view site information related to your country/region. Our Cookie Policy explains how you can opt-out of the cookies we use. If you continue without changing your cookie settings, we'll assume you’re happy with this. Bromophenol blue is 3H-2,1-Benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 3,5-dibromo-4-hydroxyphenyl groups. 2019 Mar 22; 540: 148-154. Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.[4]. Bromophenol blue (115-39-9) Chemical Names and Synonyms. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS" For licensing inquiries, please contact partnerships@abcam.com. It has also been used as an industrial d In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red colour, where the apparent colour shifts depending on … J Colloid Interface Sci. PubChem Compound. * Please select Quantity before adding items. As an acid–base indicator, its useful range lies between pH 3.0 and 4.6. Your information is safe with us. We have received your request and will respond to you as soon as possible. Your browser does not have JavaScript enabled and some parts of this website will not work without it. Thank you for being a loyal MedChemExpress customer, we are here to assist you as needed. Guaranteed product quality, expert customer support. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. [5] Mendoza L, et, al. InChI=1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H, InChI=1/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H, Brc1cc(cc(Br)c1O)C3(OS(=O)(=O)c2ccccc23)c4cc(Br)c(O)c(Br)c4, Except where otherwise noted, data are given for materials in their. We use cookies to make our site as useful as possible. Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein in a gel; the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in a 1X TAE buffer or TBE buffer, bromophenol blue migrates at the same rate as a DNA fragment of about 300 base pairs, in 2% agarose as 150 bp. As the situation with COVID-19 continues to unfold in every community, MedChemExpress is responding to the uncertainty caused by this outbreak thoughtfully and cautiously. ab146339 has not yet been referenced specifically in any publications. All rights reserved. Please refer to the solubility information to select the appropriate solvent. Please try the standard protocols listed below and let us know how you get on. Bromophenol blue is also used as a dye. Mendoza L, et, al. It changes from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible. DMSO : 50 mg/mL (74.63 mM; Need ultrasonic), Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline, Solubility: ≥ 2.5 mg/mL (3.73 mM); Clear solution, Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline). Please let us know so that we can cite the reference in this datasheet. [2]. Agonists, activators, antagonists and inhibitors. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. . Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol), Concentration (start) × Volume (start) = Concentration (final) × Volume (final), This equation is commonly abbreviated as: C1V1 = C2V2, Bromophenol blueOtherstrackingdyeelectrophoresispHindicatorInhibitorinhibitorinhibit. Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. Get resources and offers direct to your inbox. Thanks, your subscription has been confirmed. PubChem Compound. Bromophenol blue is also used as a dye. - Mechanism of Action & Protocol. We do not sell to patients. Products are chemical reagents for research use only and are not intended for human use. Dangui AZ, et, al. Store under desiccating conditions. Please visit our frequently asked questions (FAQ) page for more details. Please note: All products are "FOR RESEARCH USE ONLY. Access advice and support for any research roadblock, Full event breakdown with abstracts, speakers, registration and more, bromophenol-blue-tracking-dye-ab146339.pdf. Migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. Santa Cruz Biotechnology, Inc. Bromophenol Blue (CAS 115-39-9) Properties. Tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. Sorry, but the email address you supplied was invalid. We will send it to your Email address shortly. Nanocellulose for gel electrophoresis. At neutral pH, the dye absorbs red light most strongly and transmits blue light. Wherever possible, you should prepare and use solutions on the same day. Bromophenol blue is an acid phthalein dye, and it is used as a tracking dye for electrophoresis. https://www.scbt.com/scbt/product/bromophenol-blue-115-39-9, https://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=8272&loc=ec_rcs, http://www.sigmaaldrich.com/Graphics/COfAInfo/SigmaSAPQM/SPEC/B0/B0126/B0126-BULK________SIAL_____.pdf, "Agarose gel electrophoresis (basic method)", "Quantification of dichromatism: a characteristic of color in transparent materials", https://en.wikipedia.org/w/index.php?title=Bromophenol_blue&oldid=984535214, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 20 October 2020, at 16:16. Generally, these will be useable for up to one month. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6. In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red colour, where the apparent colour shifts depending on the concentration and/or path length through which the solution is observed. If you need to change the delivery plan for items ordered, please contact us via email [email protected]. There are currently no Customer reviews or Questions for ab146339.Please use the links above to contact us or submit feedback about this product. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel.

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