In addition, the microorganism number obtained increased by increasing the concentration of the compounds, but there was no important increase in the microorganism number at the high substrate concentration of phenol and benzoic acid. The biodegradation of 10 (■), 50 (▲), 70 (●) and 80 (♦) mg/l phenol, and the bacteria number for 10 (), 50 (∆), 70 (○) and 80 (◊) mg/l phenol. The solution sterilized to the autoclave and the incubation is done at 37˚C, the reading is done after 24 h. Bacterial growth was measured spectrophotometrically by optical density at 600 nm (OD600) [11].The transformation of DO into UFC / ml is made by the relation: Benzoic acid and phenol were used as the carbon sources, and all the solutions/media were made in double distilled. The degradation time of phenol for pH 5.6, 8 and 7 was 96 h, 120 h, 72 h respectively. This represents the first biotechnological application of a Carboxylase enzyme. The Phenol, and benzoic acid, as acids, will react with the base to form phenolate and benzenoate ions, which will dissolve into the water layer. 3, No. Hydroxy acid: An carboxylic acid containing an additional hydroxy group (-OH), substistuted to another carbon atom than the acid group. 4-OH benzoic acid has been synthesized from phenol and CO 2 at room temperature and sub-atmospheric pressure of CO 2 with 100 % selectivity, using a Carboxylase enzyme. Comparison of photocatalytic activity of undoped and doped ZnO powders demonstrates that doping with sodium and lithium increases the efficiency of degradation of both phenol and benzoic acid. 216-222. Answered By . Results obtained at the time of the different experimentations clearly show that phenol and the benzoic acid were eliminated by the Pseudomonas aeruginosa bacterium. 1-3, 2009, pp. We use cookies to help provide and enhance our service and tailor content and ads. The biodegradation of 10 (■), 50 (▲), 70 (●) and 80 (♦) mg/l benzoic acid and the bacteria number for 10 (), 50 (∆), 70 (○) and 80 (◊) mg/l benzoic acid. G. Wei, J. Yu, Y. Zhu, W. Chen and L. Wang, “Characterization of Phenol Degradation by Rhizobium Sp CCNWTB 701 Isolated from Astragalus Chrysopteru in Mining Tailing Region,” Journal of Hazardous Materials, Vol. Copyright ? However, benzoic acid is the most acidic compound among them, it can react with bicarbonate ion. F. Hamadi, H. Latrache, E. Mliji and B. mallouki, “Adhésion de staphylococus aureus au verre et au teflon,” Rcv. Compound Concentration g/l Phenol and benzoic acid Variable KH2PO4 1.7 K2HPO4 4.3 (NH4)2SO4 2.69 CaCl2 0.03 MgSO4 0.2 water. 135, No. D. F. Goerlitz, D. E. Troutman and E. M. Godsy, “Migration of Wood-Preserving Chemicals in Contaminated Groundwater in a Sand Aquifer at Pensacola,” Science &.Technology, Vol. 156-161. The biodegradation of these compounds was investigated individually using Pseudomonas aeroginosa. Microbiol, Ind , San et Environ, Vol. However, it was noted that during the various investigations the bacterium Pseudomonas aeruginosa develops better in a phenol milieu and therefore degrades phenol more than benzoic acid. Composition of the synthetic medium [10]. DJ1 Aerobic Granules,” Bioresource Technology, Vol. M. Afzal, S. Iqbal, S. Rauf and Z. M. Khalid, “Characteristics of Phenol Biodegradation in Saline Solutions by Monocultures of Pseudomonas Aeruginosa and Pseudomonas Pseudomallei,” Journal of Hazardous Materials, Vol. The results showed that the lag period, the biodegradation time, and the number of microorganism increased on increasing the substrate concentrations. After 24 hours, the mixture of every solution was centrifuged at 4000 g for 15 min; the degraded quantity of the compound was determined by the equation obtained by standardization curves. A cheap, fast and easy method for the phosphorylation of phenol has been also developed. Figure 4. Related Topics . 1, 2009, pp. Journal of Water Resource and Protection Vol. Many aerobic phenol-degrading microorganisms were isolated and the pathways for the aerobic degradation of phenol are now definitely well established. The results for effect of pH on phenol and benzoic acid biodegradation experiments are shown in Figures 3,4 respectively. Doping ZnO with lithium, sodium and potassium increases the crystallinity of powders and reduces band gap energy. Benzoic acid is a common additive for preserving foods, fats, fruit juices, alkaloid solutions, and curing tobacco. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Photodegradation of phenol and benzoic acid by sol–gel-synthesized alkali metal-doped ZnO. A cheap, fast and easy method for the phosphorylation of phenol has been also developed. The in vitro percutaneous absorption of three model compounds, nicotinic acid, phenol and benzoic acid, and the herbicide triclopyr butoxyethyl ester (triclopyr BEE) has been investigated in flow-through diffusion cells using skin from male Fischer 344 rats and humans. Therefore, a large amount of phenol and phenolic compounds are effused into stream and soil, and have badly polluted the environment [1]. 1, 2008, pp. 4-OH benzoic acid has been synthesized from phenol and CO2 at room temperature and sub-atmospheric pressure of CO2 with 100 % selectivity, using a Carboxylase enzyme. Composition of the synthetic medium [10]. We notice that Pseudomonas aeruginosa totally destroyed the phenol and benzoic acid quantity in a short degradation time at PH = 7. Phenol and benzoic acid degradation is faster at PH = 7 because it is the appropriate pH for the growth of the Pseudomonas aeruginosa bacterium. 2.4. Phenol hydroxylases ranging from simple flavoprotein monooxygenases to multi component hydroxylases, as well as the genes coding for these enzymes, were described for few aerobic phenol-degrading microorganisms [9]. 8, 2009, pp. 1-3, 2006, pp. After that, volume of every solution was 50 ml, which we mixed with 0.047.108 UFC/ml of Pseudomonas aeruginosa and submitted to agitation and incubation for 24 h. After the biodegradation of 10, 50, 70 and 80 mg/l phenol 10, 50, 70 and 80 mg/l benzoic acid were conducted, the results obtained for phenol and benzoic acid biodegradation experiments are shown in Figures 1,2 respectively.

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