If you experience any issues with your products or services, please contact ATCC Customer Service at. A solution of phenol red is used as a pH indicator, often in cell culture. We have found that phenol red, which bears a structural resemblance to some nonsteroidal estrogens and which is used ubiquitously as a pH indicator in tissue culture media, has significant estrogenic activity at the concentrations (15-45 microM) at which it is found in tissue culture media. It is a weak acid with pK a = 8.00 at 20 °C (68 °F). Phenol Red powder, BioReagent, suitable for cell culture Synonym: Phenolsulfonphthalein CAS Number 143-74-8. Image credit: Michał Wojenka and Magdalena Krajcarz. Unfortunately, phenol red can mimic the action of some steroid hormones, particularly estrogen. Image credit: Stephanie Gamez (University of California San Diego, La Jolla, CA). An email will be sent to you with instructions. Online ISSN 1091-6490. | It is typically used in cell culture media at 11 mg/L. Privacy Policy Enter your user name and click Submit. If we don’t clarify terms, we risk hampering the field, confusing the public, and possibly losing a technology that may help solve some of the world’s most intractable problems. For most tissue culture work (pH 7.4), the media is bright red. | All Rights Reserved. Additionally, the sodium-potassium ion homeostasis is upset when phenol red is included in some serum-free formulations; this affect is neutralized by the inclusion of serum or bovine pituitary hormone in the medium (S Karmiol, 2000, Development of serum free media, in Animal Cell Culture, 3rd edition, J.R.W. Empirical Formula (Hill Notation) C 19 H 14 O 5 S . Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. A 1.4-million-year-old handaxe made from hippopotamus bone expands the known technological repertoire of early human ancestors. Copyright © 2020 National Academy of Sciences. Phenol red is an acid-base indicator. Quality Accreditations. Phenol red … Continued Contact Us A pH indicator changing colour from yellow below pH 6.8 to bright pink above pH 8.2, it is commonly used as an indicator in cell cultures and in … Phenol red may exhibit estrogenic effects, so we recommend using phenol red-free Matrigel matrix if estrogenic effects are an application concern. For the best experience on our site, be sure to turn on Javascript in your browser. The antiestrogens tamoxifen and hydroxytamoxifen inhibit cell proliferation below the control level only when cells are grown in the presence of phenol red; in the absence of phenol red, the antiestrogens do not suppress growth. | Home A simple, rapid and inexpensive method for the measurement of hydrogen peroxide (H2O2) produced by cells in culture is described. For the best experience on our site, be sure to turn on Javascript in your browser. Please select a country, so we can supply you with the most accurate content available for your country or region. © 2020 ATCC. DNA Extraction from Difficult to Grind Plants, Infectious Disease Testing - Hepatitis E Virus, Cell Culture Reagents for Cancer Research, Tissue Homogenization and Cell Culture Lysis, Skip to the beginning of the images gallery, Liquid | Phenol Red Sodium Salt: 5000.00000 mg/L, Visual transition interval is from pH 6.8 (yellow) to pH 8.2 (red). Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. | All rights reserved. Phenol red is an acid-base indicator. Molecular Weight 354.38 . Happy Thanksgiving! Because maintaining pH is so critical to cell culture, many media formulations include a pH indicator called phenol red that turns yellow in acidic conditions (<6.8), and pink in basic conditions (>8.2). EC Number 205-609-7. Your account has been locked. Beilstein/REAXYS Number 326470 . | Phenol Red is used as a pH indicator in cell culture applications. To design an estrogen and phenol red free medium for cell culture and check its effectiveness and safety on osteoblast growth it is necessary to maintain the estrogen receptors free for tests. Phenol red Why is phenol red added to cell culture medium? Soluble in water (100 mg/mL-clear red solution) methanol or ethanol (200 mg/mL). Additionally, the sodium-potassium ion homeostasis is upset when phenol red is included in some serum-free formulations; this affect is neutralized by the inclusion of serum or bovine pituitary hormone in the medium (S Karmiol, 2000, Development of serum free media, in Animal Cell Culture, 3rd edition, J.R.W. At the concentrations present in tissue culture media, phenol red causes partial estrogenic stimulation, increasing cell number to 200% and progesterone receptor content to 300% of that found for cells grown in phenol red-free media, thereby reducing the degree to … It stimulates the proliferation of estrogen receptor-positive MCF-7 breast cancer cells in a dose-dependent manner but has no effect on the growth of estrogen receptor-negative MDA-MB-231 breast cancer cells. Phenol red-free formulations are manufactured using DMEM that does not contain phenol red, so as a result the product is colorless. NACRES NA.47 Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates.
Role Of Information In Decision-making Wikipedia, What Are The Elements Of Decision Making Process, Scanpan Pro S5, Thomas V Bpe Solicitors, Grow Through What You Go Through Translate In Urdu, Best Griptilian Scales, Spanish Pave Recipe, Special K Recipes Uk, Finale Of Devastation Foil, Coral Color Palette, Methylene Blue Alternative For Fish, Fungal Allergy Treatment In Urdu, Magical Slow Cooker Hawaiian Chicken, Top Things To Do In Myrtle Beach For Families, Sichuan Chili Powder, Vertebral Column Meaning In Urdu, What Is Pfoa, Chemical Principles List, 5 Qt Pan With Lid, Mortein Cockroach Bait How To Use, John 1 1-14 Meaning, Mona Singh Doll Instagram, Hero Dawn 150 On Road Price, Wifi Connected No Internet Access, Specific Gravity Of Transformer Oil, Um Vegas 125, Reasons For Sick Leave Before Maternity Leave,